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PD-1/CD279 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 31.25-2000 pg/mL

Cat No : MP00612-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00612-4, Human PD-1/CD279 Recombinant Matched Antibody Pair - PBS only. 83632-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0974. 83632-4-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL

    Sandwich ELISA standard curve of MP00612-4

    Sandwich ELISA standard curve of MP00612-4, Human PD-1/CD279 Recombinant Matched Antibody Pair - PBS only. 83632-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0974. 83632-4-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL

  • Serum of sixteen individual healthy human donors was measured. The human PD-1/CD279 concentration of detected samples was determined to be 1,025.5 pg/mL with a range of 265.2 - 2,943.1 pg/mL.

    Sample test of MP00612-4

    Serum of sixteen individual healthy human donors was measured. The human PD-1/CD279 concentration of detected samples was determined to be 1,025.5 pg/mL with a range of 265.2 - 2,943.1 pg/mL.

  • For the PBMC supernatant cultured for 5 days, the mean human PD-1/CD279 concentration was determined to be 382.5 pg/mL in PHA stimulated PBMC supernatant.

    Sample test of MP00612-4

    For the PBMC supernatant cultured for 5 days, the mean human PD-1/CD279 concentration was determined to be 382.5 pg/mL in PHA stimulated PBMC supernatant.

  • Urine of eight individual healthy human was measured. The PD-1/CD279 concentration of detected samples was determined to be 856.9 pg/mL with a range of 280.3 - 1,536.5 pg/mL.

    Sample test of MP00612-4

    Urine of eight individual healthy human was measured. The PD-1/CD279 concentration of detected samples was determined to be 856.9 pg/mL with a range of 280.3 - 1,536.5 pg/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00612-4 targets PD-1/CD279 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 31.25-2000 pg/mL (Sandwich ELISA);

Capture antibody: PD-1/CD279 Recombinant antibody, PBS Only (Capture) 83632-5-PBS (240724C5). 100 μg. Concentration 1 mgl/ml.

Detection antibody: PD-1/CD279 Recombinant antibody, PBS Only (Detector) 83632-4-PBS (240724G9). 100 μg. Concentration 1 mgl/ml.

Alternative PD-1/CD279 matched antibody pairs: MP00612-1, MP00612-2, MP00612-3

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83632-5-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name programmed cell death 1
Synonyms CD279, PD1, PD-1, 240724C5, PD 1
GenBank accession numberBC074740
Gene symbol PD-1
UniprotQ15116
Gene ID (NCBI) 5133
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 83632-4-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name programmed cell death 1
Synonyms CD279, PD1, PD-1, 240724G9, PD 1
GenBank accession numberBC074740
Gene symbol PD-1
UniprotQ15116
Gene ID (NCBI) 5133
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C