• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Human PD-L1 (B7-H1) (29E.2A3)

PD-L1 (B7-H1) Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human and More (1)

Applications

FC

Conjugate

Unconjugated

CloneNo.

29E.2A3

Cat no : 65081-1-Ig

Print datasheet

Synonyms

CD274, PD-L1, PD L1, hPD-L1, B7-H1

验证数据展示

  • FC experiment of human PBMCs using 65081-1-Ig

    FC experiment of human PBMCs using 65081-1-Ig

    1X10^6 PHA treated (3d) human PBMCs were surface stained with 0.2 ug Anti-Human PD-L1 (B7-H1) (65081-1-Ig, Clone:29E.2A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or Mouse IgG2b Isotype Control (blue). 1X10^6 untreated human PBMCs were surface stained with 0.2 ug Anti-Human PD-L1 (B7-H1) (65081-1-Ig, Clone:29E.2A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black dashed). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65081-1-Ig

    FC experiment of human PBMCs using 65081-1-Ig

    1X10^6 PHA treated (3d) human PBMCs were surface stained with 0.2 ug Anti-Human PD-L1 (B7-H1) (65081-1-Ig, Clone:29E.2A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (right), or Mouse IgG2b Isotype Control (left). 1X10^6 untreated human PBMCs were surface stained with 0.2 ug Anti-Human PD-L1 (B7-H1) (65081-1-Ig, Clone:29E.2A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (center). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inPHA treated human PBMCs
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65081-1-Ig targets PD-L1 (B7-H1) in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen Full length human PD-L1 种属同源性预测
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name CD274 molecule
Synonyms CD274, PD-L1, PD L1, hPD-L1, B7-H1
GenBank Accession NumberBC069381
Gene Symbol PD-L1
Gene ID (NCBI) 29126
RRIDAB_2918384
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ9NZQ7
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

实验方案

Product Specific Protocols
FC protocol for PD-L1 (B7-H1) antibody 65081-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Transl Res

Immune checkpoint activity exacerbate renal interstitial fibrosis progression by enhancing PD-L1 expression in renal tubular epithelial cells

Authors - Yuting Zhang
  • KD Validated
View Article