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Cardinal Red™ Anti-Human PD-L1 (B7-H1) (29E.2A3)

PD-L1 (B7-H1) Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

FC

Conjugate

Cardinal Red™ Fluorescent Dye

CloneNo.

29E.2A3

Cat no : CR-65081

Print datasheet

Synonyms

CD274, PD-L1, PD L1, hPD-L1, B7-H1

验证数据展示

  • FC experiment of human PBMCs using CR-65081

    FC experiment of human PBMCs using CR-65081

    1x10^6 PHA treated (3 days) human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human PD-L1 (B7-H1) (CR-65081, Clone:29E.2A3) (red) or Cardinal Red™ Mouse IgG2b Isotype Control (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human PD-L1 (B7-H1) (CR-65081, Clone:29E.2A3) (black dashed). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CR-65081

    FC experiment of human PBMCs using CR-65081

    1x10^6 PHA treated (3 days) human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul Cardinal Red™ Anti-Human PD-L1 (B7-H1) (CR-65081, Clone:29E.2A3) (right) or Cardinal Red™ Mouse IgG2b Isotype Control (left). 1x10^6 untreated human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human PD-L1 (B7-H1) (CR-65081, Clone:29E.2A3) (center). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inPHA treated human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CR-65081 targets PD-L1 (B7-H1) in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Full length human PD-L1 种属同源性预测
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name CD274 molecule
Synonyms CD274, PD-L1, PD L1, hPD-L1, B7-H1
GenBank Accession NumberBC069381
Gene Symbol PD-L1
Gene ID (NCBI) 29126
Conjugate Cardinal Red™ Fluorescent Dye
Excitation/Emission Maxima Wavelengths592 nm / 611 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ9NZQ7
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

实验方案

Product Specific Protocols
FC protocol for Cardinal Red™ PD-L1 (B7-H1) antibody CR-65081Download protocol
Standard Protocols
Click here to view our Standard Protocols