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验证数据展示

  • Sandwich ELISA standard curve of MP00601-4

    Sandwich ELISA standard curve of MP00601-4

    Sandwich ELISA standard curve of MP00601-4, Human PD-L1/CD274 Recombinant Matched Antibody Pair - PBS only. 83600-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1114. 83600-6-PBS was HRP conjugated as the detection antibody. Range: 3.9-250 pg/mL.

  • Sample test of MP00601-4

    Sample test of MP00601-4

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days or 5 days. For the PBMC supernatant cultured for 3 days, the mean PD-L1/CD274 concentration was determined to be 7.7 pg/mL in unstimulated PBMC supernatant, 108.4 pg/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean PD-L1/CD274 concentration was determined to be 11.0 pg/mL in unstimulated PBMC supernatant, 160.7 pg/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP00601-4

    Sample test of MP00601-4

    Urine of seven individual healthy human donors was measured. The PD-L1/CD274 concentration of detected samples was determined to be 10.8 pg/mL with a range of 0.7 - 33.1 pg/mL.

  • Sample test of MP00601-4

    Sample test of MP00601-4

    The mean PD-L1/CD274 concentration was determined to be 36,368.3 pg/mL in MDA-MB-231 cell extract based on a 2.3 mg/mL extract load.

  • Sample test of MP00601-4

    Sample test of MP00601-4

    Serum of sixteen individual healthy human donors was measured. The PD-L1/CD274 concentration of detected samples was determined to be 75.1 pg/mL with a range of 34.4-168.1 pg/mL.

  • Cytometric bead array standard curve of MP00601-3

    Cytometric bead array standard curve of MP00601-3

    Cytometric bead array standard curve of MP00601-3, PD-L1/CD274 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83600-6-PBS. Detection antibody: 83600-4-PBS. Standard: Eg1114. Range: 0.156-20 ng/mL.

PD-L1/CD274 Recombinant antibody, PBS Only (Capture/Detector)

PD-L1/CD274 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83600-6-PBS

产品说明书

CloneNo. 240721D2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

CD274, PD-L1, PD L1, hPD-L1, B7-H1

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83600-6-PBS targets PD-L1/CD274 as part of a matched antibody pair:

MP00601-3: 83600-6-PBS capture and 83600-4-PBS detection (validated in Cytometric bead array)

MP00601-4: 83600-7-PBS capture and 83600-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD274 molecule
别名 CD274, PD-L1, PD L1, hPD-L1, B7-H1
计算分子量 290 aa, 33 kDa
GenBank蛋白编号BC074984
基因名称 PD-L1
Gene ID (NCBI) 29126
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NZQ7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C