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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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验证数据展示

  • FC experiment of mouse splenocytes using 98166-1-RR (same clone as 98166-1-PBS)

    FC experiment of mouse splenocytes using 98166-1-RR (same clone as 98166-1-PBS)

    1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/CD274 Rabbit Recombinant Antibody (98166-1-RR, Clone: 240993E1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD3. Cells were not fixed. This data was developed using the same antibody clone with 98166-1-PBS in a different storage buffer formulation.

  • FC experiment of mouse splenocytes using 98166-1-RR (same clone as 98166-1-PBS)

    FC experiment of mouse splenocytes using 98166-1-RR (same clone as 98166-1-PBS)

    1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/B7-H1 Rabbit Recombinant Antibody (98166-1-RR, Clone: 240993E1) (red) or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/B7-H1 Rabbit Recombinant Antibody (98166-1-RR, Clone: 240993E1) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98166-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98166-1-RR

    Affinity and Kinetic Characterization of 98166-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98166-1-RR against Mouse PD-L1/CD274 were performed. The affinity constant is 2.24 nM.

Anti-Mouse PD-L1/CD274 Rabbit Recombinant Antibody, PBS Only

PD-L1/CD274 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98166-1-PBS

产品说明书

CloneNo. 240993E1

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

Cd274, PD-L1, Pdcd1lg1, Pdcd1l1, PD L1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

98166-1-PBS targets PD-L1/CD274 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD274 antigen
别名 Cd274, PD-L1, Pdcd1lg1, Pdcd1l1, PD L1
计算分子量33 kDa
GenBank蛋白编号NM_021893.3
基因名称 PD-L1
Gene ID (NCBI) 60533
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9EP73
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.