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Anti-Mouse PD-L1/CD274 Rabbit Recombinant Antibody, PBS Only

PD-L1/CD274 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240993E1

Cat no : 98166-1-PBS

Print datasheet

Synonyms

Cd274, PD-L1, Pdcd1lg1, Pdcd1l1, PD L1

验证数据展示

  • FC experiment of mouse splenocytes using 98166-1-RR (same clone as 98166-1-PBS)

    FC experiment of mouse splenocytes using 98166-1-RR (same clone as 98166-1-PBS)

    1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/CD274 Rabbit Recombinant Antibody (98166-1-RR, Clone: 240993E1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD3. Cells were not fixed. This data was developed using the same antibody clone with 98166-1-PBS in a different storage buffer formulation.

  • FC experiment of mouse splenocytes using 98166-1-RR (same clone as 98166-1-PBS)

    FC experiment of mouse splenocytes using 98166-1-RR (same clone as 98166-1-PBS)

    1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/B7-H1 Rabbit Recombinant Antibody (98166-1-RR, Clone:240993E1) (red) or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/B7-H1 Rabbit Recombinant Antibody (98166-1-RR, Clone: 240993E1) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98166-1-PBS in a different storage buffer formulation.

产品信息

98166-1-PBS targets PD-L1/CD274 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD274 antigen
Synonyms Cd274, PD-L1, Pdcd1lg1, Pdcd1l1, PD L1
Calculated Molecular Weight33 kDa
GenBank Accession NumberNM_021893.3
Gene Symbol PD-L1
Gene ID (NCBI) 60533
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9EP73
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.