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Anti-Mouse PD-L1/CD274 Rabbit Recombinant Antibody

PD-L1/CD274 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240993E1

Cat no : 98166-1-RR

Print datasheet

Synonyms

Cd274, PD-L1, Pdcd1lg1, Pdcd1l1, PD L1

验证数据展示

  • FC experiment of mouse splenocytes using 98166-1-RR

    FC experiment of mouse splenocytes using 98166-1-RR

    1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/CD274 Rabbit Recombinant Antibody (98166-1-RR, Clone: 240993E1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD3. Cells were not fixed.

  • FC experiment of mouse splenocytes using 98166-1-RR

    FC experiment of mouse splenocytes using 98166-1-RR

    1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/B7-H1 Rabbit Recombinant Antibody (98166-1-RR, Clone:240993E1) (red) or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/B7-H1 Rabbit Recombinant Antibody (98166-1-RR, Clone: 240993E1) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

经过测试的应用

Positive FC detected inanti-CD3/CD28 treated mouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98166-1-RR targets PD-L1/CD274 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD274 antigen
Synonyms Cd274, PD-L1, Pdcd1lg1, Pdcd1l1, PD L1
Calculated Molecular Weight33 kDa
GenBank Accession NumberNM_021893.3
Gene Symbol PD-L1
Gene ID (NCBI) 60533
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9EP73
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

实验方案

Product Specific Protocols
FC protocol for PD-L1/CD274 antibody 98166-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols