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PDAP1 Polyclonal antibody

PDAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat No : 15081-1-AP

Print datasheet

Synonyms

PDGF-associated protein, PDGFA-associated protein 1, PDGFA associated protein 1, PDGF associated protein, HASPP28

验证数据展示

  • WB analysis of MCF-7 using 15081-1-AP

    WB analysis of MCF-7 using 15081-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15081-1-AP (PDAP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15081-1-AP

    IF Staining of HeLa using 15081-1-AP

    Immunofluorescent analysis of Hela cells, using PDAP1 antibody 15081-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human breast cancer using 15081-1-AP

    IHC staining of human breast cancer using 15081-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15081-1-AP (PDAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDAP1 antibody included
  • IHC staining of human breast cancer using 15081-1-AP

    IHC staining of human breast cancer using 15081-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15081-1-AP (PDAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDAP1 antibody included
  • IP experiment of MCF-7 using 15081-1-AP

    IP experiment of MCF-7 using 15081-1-AP

    IP result of anti-PDAP1 (IP:15081-1-AP, 4ug; Detection:15081-1-AP 1:1000) with MCF-7 cells lysate 2400ug.

经过测试的应用

Positive WB detected inMCF7 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells
Planning an IHC experiment? We recommend our IHCeasy PDAP1 Ready-To-Use IHC Kit. PDAP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15081-1-AP targets PDAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen PDAP1 fusion protein Ag7155 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PDGFA associated protein 1
Synonyms PDGF-associated protein, PDGFA-associated protein 1, PDGFA associated protein 1, PDGF associated protein, HASPP28
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC000684
Gene Symbol PDAP1
Gene ID (NCBI) 11333
RRIDAB_2159306
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13442
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDAP1 antibody 15081-1-APDownload protocol
IHC protocol for PDAP1 antibody 15081-1-APDownload protocol
IF protocol for PDAP1 antibody 15081-1-APDownload protocol
IP protocol for PDAP1 antibody 15081-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Proteomics Clin Appl

SILAC-based quantitative proteomic analysis of gastric cancer secretome.

Authors - Marimuthu Arivusudar A
View Article
humanWB

Cancer Sci

Pancreatic ductal adenocarcinoma cells regulated the gemcitabine-resistance function of CAFs by LINC00460

Authors - Xiao‐Xu Zhu
View Article