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CoraLite® Plus 488-conjugated PDAP1 Polyclonal antibody

PDAP1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15081

Print datasheet

Synonyms

PDGF-associated protein, PDGFA-associated protein 1, PDGFA associated protein 1, PDGF associated protein, HASPP28

验证数据展示

  • FC experiment of MCF-7 using CL488-15081

    FC experiment of MCF-7 using CL488-15081

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Pdap1 Polyclonal Antibody (CL488-15081)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PDAP1 Ready-To-Use IHC Kit. PDAP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15081-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15081 targets PDAP1 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen PDAP1 fusion protein Ag7155 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PDGFA associated protein 1
Synonyms PDGF-associated protein, PDGFA-associated protein 1, PDGFA associated protein 1, PDGF associated protein, HASPP28
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC000684
Gene Symbol PDAP1
Gene ID (NCBI) 11333
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13442
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PDAP1 antibody CL488-15081Download protocol
Standard Protocols
Click here to view our Standard Protocols