验证数据展示
经过测试的应用
| Positive WB detected in | A2780 cells, COLO 320 cells, MCF-7 cells |
| Positive IF/ICC detected in | HepG2 cells, MCF-7 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
14997-1-AP targets PDCL3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | PDCL3 fusion protein Ag6983 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosducin-like 3 |
| 别名 | Phosducin-like protein 3, Phosducin like protein 3, phosducin like 3, PHLP3, PhLP2A |
| 计算分子量 | 28 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC001021 |
| 基因名称 | PDCL3 |
| Gene ID (NCBI) | 79031 |
| RRID | AB_2878098 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H2J4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDCL3 antibody 14997-1-AP | Download protocol |
| IF protocol for PDCL3 antibody 14997-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
