• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PDCL3 Polyclonal antibody

PDCL3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14997-1-AP

Print datasheet

Synonyms

Phosducin-like protein 3, Phosducin like protein 3, phosducin like 3, PHLP3, PhLP2A

验证数据展示

  • WB analysis using 14997-1-AP

    WB analysis using 14997-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14997-1-AP (PDCL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 14997-1-AP

    WB analysis using 14997-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14997-1-AP (PDCL3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 14997-1-AP

    IF Staining of HepG2 using 14997-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PDCL3 antibody (14997-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA2780 cells, COLO 320 cells, MCF-7 cells
Positive IF/ICC detected inHepG2 cells, MCF-7 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14997-1-AP targets PDCL3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen PDCL3 fusion protein Ag6983 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosducin-like 3
Synonyms Phosducin-like protein 3, Phosducin like protein 3, phosducin like 3, PHLP3, PhLP2A
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC001021
Gene Symbol PDCL3
Gene ID (NCBI) 79031
RRIDAB_2878098
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2J4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDCL3 antibody 14997-1-APDownload protocol
IF protocol for PDCL3 antibody 14997-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Eur J Med Res

PDCL3 is a prognostic biomarker associated with immune infiltration in hepatocellular carcinoma

Authors - Wenzhi Jin
View Article