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验证数据展示

  • WB analysis using 84869-1-RR

    WB analysis using 84869-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84869-1-RR (PDE10A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 84869-1-RR

    FC experiment of HeLa using 84869-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PDE10A Recombinant antibody (84869-1-RR, Clone:242357E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84869-1-RR

    Affinity and Kinetic Characterization of 84869-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84869-1-RR against Human PDE10A were performed. The affinity constant is 3.37 nM.

PDE10A Recombinant antibody

PDE10A Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84869-1-RR

产品说明书

CloneNo. 242357E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

phosphodiesterase 10A, HSPDE10A, EC:3.1.4.17, cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A, 242357E12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84869-1-RR targets PDE10A in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PDE10A fusion protein Ag12771 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphodiesterase 10A
别名 phosphodiesterase 10A, HSPDE10A, EC:3.1.4.17, cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A, 242357E12
计算分子量 779 aa, 88 kDa
观测分子量80 kDa
GenBank蛋白编号BC104860
基因名称 PDE10A
Gene ID (NCBI) 10846
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y233
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDE10A (Phosphodiesterase 10A) is a single member of the PDE10 protein family, and it catalyzes both cAMP and cGMP hydrolysis (PMID: 34550322). The literature on PDE10A has been focused on psychiatric and neurological disorders because PDE10A expression is enriched in the striatal region of the brain under normal conditions (PMID: 12967715). Moreover, PDE10A expression is induced in various human tumor cell lines and PDE10A inhibition suppresses tumor cell growth (PMID: 37232184).

实验方案

Product Specific Protocols
WB protocol for PDE10A antibody 84869-1-RRDownload protocol
FC protocol for PDE10A antibody 84869-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols