验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 12918-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-12918 targets PDE4D in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | PDE4D fusion protein Ag3588 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) |
| Synonyms | DPDE3, HSPDE4D, PDE43, PDE4D, PDE4DN2, STRK1 |
| Calculated Molecular Weight | 809 aa, 91 kDa |
| Observed Molecular Weight | 91 kDa |
| GenBank Accession Number | BC036319 |
| Gene Symbol | PDE4D |
| Gene ID (NCBI) | 5144 |
| RRID | AB_3083933 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08499 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDE4D(cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also named as DPDE3, PDE43 and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram(PMID:16730340). It has some isoforms and all the PDE4D isoforms are expressed in healthy adult human hippocampus and in hippocampus from a patient with advanced Alzheimer disease(PMID:17353396).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PDE4D antibody CL488-12918 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |