验证数据展示
经过测试的应用
| Positive WB detected in | mouse eye tissue, rat eye tissue, mouse retina tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67832-1-Ig targets PDE6A in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | Human, Mouse, Rat |
| Immunogen | PDE6A fusion protein Ag16741 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | phosphodiesterase 6A, cGMP-specific, rod, alpha |
| Synonyms | CGPR A, GMP PDE alpha, PDE V B1, PDE6 alpha, PDE6A, PDEA |
| Calculated Molecular Weight | 860 aa, 100 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC035909 |
| Gene Symbol | PDE6A |
| Gene ID (NCBI) | 5145 |
| RRID | AB_2918594 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P16499 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDE6A(Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha) is also named as GMP-PDE alpha, PDE V-B1, PDEA and belongs to the cyclic nucleotide phosphodiesterase family. PDE6A contains an open reading frame capable of coding for a polypeptide of 859 amino acids and about 100 kDa. It is a subunit of a key phototransduction enzyme which participates in processes of transmission and amplification of the visual signal. The expression of PDE6 alpha in retina is essential for normal expression of PDE6 beta and PDE6 gama.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDE6A antibody 67832-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
