验证数据展示
经过测试的应用
| Positive WB detected in | mouse eye tissue, rat eye tissue, mouse retina tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67832-1-Ig targets PDE6A in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, Mouse, Rat |
| 免疫原 | PDE6A fusion protein Ag16741 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | phosphodiesterase 6A, cGMP-specific, rod, alpha |
| 别名 | CGPR A, GMP PDE alpha, PDE V B1, PDE6 alpha, PDE6A, PDEA |
| 计算分子量 | 860 aa, 100 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC035909 |
| 基因名称 | PDE6A |
| Gene ID (NCBI) | 5145 |
| RRID | AB_2918594 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P16499 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDE6A(Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha) is also named as GMP-PDE alpha, PDE V-B1, PDEA and belongs to the cyclic nucleotide phosphodiesterase family. PDE6A contains an open reading frame capable of coding for a polypeptide of 859 amino acids and about 100 kDa. It is a subunit of a key phototransduction enzyme which participates in processes of transmission and amplification of the visual signal. The expression of PDE6 alpha in retina is essential for normal expression of PDE6 beta and PDE6 gama.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDE6A antibody 67832-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
