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PDGFRL Polyclonal antibody

PDGFRL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16217-1-AP

Print datasheet

Synonyms

PRLTS, Platelet-derived growth factor receptor-like protein, PDGRL, PDGFR-like protein, PDGFR like protein

验证数据展示

  • WB analysis of L02 using 16217-1-AP

    WB analysis of L02 using 16217-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 16217-1-AP (PDGFRL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 16217-1-AP

    IHC staining of human liver cancer using 16217-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16217-1-AP (PDGFRL Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PDGFRL antibody included
  • IHC staining of human liver cancer using 16217-1-AP

    IHC staining of human liver cancer using 16217-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16217-1-AP (PDGFRL Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with PDGFRL antibody included
  • IF Staining of L02 using 16217-1-AP

    IF Staining of L02 using 16217-1-AP

    Immunofluorescent analysis of L02 cells using 16217-1-AP (PDGFRL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inL02 cells, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells
Planning an IHC experiment? We recommend our IHCeasy PDGFRL Ready-To-Use IHC Kit. PDGFRL primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16217-1-AP targets PDGFRL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PDGFRL fusion protein Ag9218 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name platelet-derived growth factor receptor-like
Synonyms PRLTS, Platelet-derived growth factor receptor-like protein, PDGRL, PDGFR-like protein, PDGFR like protein
Calculated Molecular Weight 375 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC010927
Gene Symbol PDGFRL
Gene ID (NCBI) 5157
RRIDAB_2878230
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15198
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDGFRL antibody 16217-1-APDownload protocol
IHC protocol for PDGFRL antibody 16217-1-APDownload protocol
IF protocol for PDGFRL antibody 16217-1-APDownload protocol
FC protocol for PDGFRL antibody 16217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

A single cell atlas of frozen shoulder capsule identifies features associated with inflammatory fibrosis resolution

Authors - Michael T H Ng
View Article
humanWB

Aging (Albany NY)

Identification of a unique stress response state of T cells-related gene signature in patients with gastric cancer

Authors - Qin Yang
  • KD Validated
View Article
humanIHC

Mol Carcinog

The cumulative antitumor effects of regorafenib and radiotherapy in hepatocellular carcinoma

Authors - Shuwen Kuang
View Article