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  • Featured Product
  • KD/KO Validated

PDH E1 Alpha Monoclonal antibody, PBS Only

PDH E1 Alpha Monoclonal Antibody for WB,IP,Indirect ELISA,IHC,IF,FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB,IP,Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

2B3C10

Cat No : 66119-1-PBS

Print datasheet

Synonyms

PDH E1 Alpha, PDH E1α, PDHA1, PDHE1 A type I, pyruvate dehydrogenase E1 α subunit

验证数据展示

  • WB analysis of HEK293 cells using 66119-1-Ig (same clone as 66119-1-PBS)

    WB analysis of HEK293 cells using 66119-1-Ig (same clone as 66119-1-PBS)

    WB result of PDHA1 antibody (66119-1-Ig, 1:30000) with si-control and si-PDHA1 transfected HEK293 cells. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66119-1-Ig (same clone as 66119-1-PBS)

    WB analysis of HEK-293 using 66119-1-Ig (same clone as 66119-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66119-1-Ig (PDH E1α antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 66119-1-Ig (same clone as 66119-1-PBS)

    IP experiment of HEK-293 using 66119-1-Ig (same clone as 66119-1-PBS)

    IP result of anti-PDH E1 Alpha (IP:66119-1-Ig, 3ug; Detection:66119-1-Ig 1:10000) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66119-1-Ig (same clone as 66119-1-PBS)

    IHC staining of human liver cancer using 66119-1-Ig (same clone as 66119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66119-1-Ig (same clone as 66119-1-PBS)

    IHC staining of human liver cancer using 66119-1-Ig (same clone as 66119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66119-1-Ig (same clone as 66119-1-PBS)

    IF Staining of HepG2 using 66119-1-Ig (same clone as 66119-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66119-1-Ig (PDH E1α antibody) at dilution of 1:100 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66119-1-Ig (same clone as 66119-1-PBS)

    FC experiment of HepG2 using 66119-1-Ig (same clone as 66119-1-PBS)

    1X10^6 HepG2 cells were stained with .2ug PDH E1α antibody (66119-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

产品信息

66119-1-PBS targets PDH E1 Alpha in WB, IP, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB,IP,Indirect ELISA,IHC,IF,FC Application Description
Tested Reactivity human
Immunogen PDH E1 Alpha fusion protein Ag12556 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name pyruvate dehydrogenase (lipoamide) alpha 1
Synonyms PDH E1 Alpha, PDH E1α, PDHA1, PDHE1 A type I, pyruvate dehydrogenase E1 α subunit
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC002406
Gene Symbol PDHA1
Gene ID (NCBI) 5160
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08559
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).