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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated PDH E1 Alpha Monoclonal antibody

PDH E1 Alpha Monoclonal Antibody for WB, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2B3C10

Cat no : CL594-66119

Print datasheet

Synonyms

PDH E1α, PDHA1, PDHA 1, PDHA, EC:1.2.4.1

验证数据展示

  • WB analysis using CL594-66119

    WB analysis using CL594-66119

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-66119 (PDH E1 Alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using CL594-66119

    FC experiment of HepG2 using CL594-66119

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PDH E1 Alpha (CL594-66119, Clone:2B3C10) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PDHA1 Ready-To-Use IHC Kit. PDHA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66119-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66119 targets PDH E1 Alpha in WB, FC (Intra) applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human
Immunogen PDH E1 Alpha fusion protein Ag12556 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name pyruvate dehydrogenase (lipoamide) alpha 1
Synonyms PDH E1α, PDHA1, PDHA 1, PDHA, EC:1.2.4.1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC002406
Gene Symbol PDHA1
Gene ID (NCBI) 5160
RRIDAB_2919949
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08559
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).

实验方案

Product Specific Protocols
WB protocol for CL594 PDH E1 Alpha antibody CL594-66119Download protocol
FC protocol for CL594 PDH E1 Alpha antibody CL594-66119Download protocol
Standard Protocols
Click here to view our Standard Protocols