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PDHB Monoclonal antibody

PDHB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A7C10

Cat no : 68238-1-Ig

Print datasheet

Synonyms

PHE1B, PDHE1-B, PDHE1B, EC:1.2.4.1, 2A7C10

验证数据展示

  • WB analysis using 68238-1-Ig

    WB analysis using 68238-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68238-1-Ig (PDHB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 68238-1-Ig

    IHC staining of mouse kidney using 68238-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDHB antibody included
  • IF Staining of HeLa using 68238-1-Ig

    IF Staining of HeLa using 68238-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDHB antibody (68238-1-Ig, Clone: 2A7C10 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, HSC-T6 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, mouse heart tissue
Positive IHC detected inmouse kidney tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PDHB Ready-To-Use IHC Kit. PDHB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68238-1-Ig targets PDHB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen PDHB fusion protein Ag6857 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name pyruvate dehydrogenase (lipoamide) beta
Synonyms PHE1B, PDHE1-B, PDHE1B, EC:1.2.4.1, 2A7C10
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC000439
Gene Symbol PDHB
Gene ID (NCBI) 5162
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP11177
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDHB (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial) is also named PHE1B. The enzyme, which is found in mitochondria, is one of the component enzymes of the pyruvate dehydrogenase multienzyme complex (PDH). It catalyzes the first reaction of an oxidative decarboxylation sequence converting pyruvate to acetyl-CoA and CO(2)(PMID:3422424). Defects in PDHB are the cause of pyruvate dehydrogenase E1-beta deficiency (PDHBD)(PMID:15138885).

实验方案

Product Specific Protocols
WB protocol for PDHB antibody 68238-1-IgDownload protocol
IHC protocol for PDHB antibody 68238-1-IgDownload protocol
IF protocol for PDHB antibody 68238-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols