• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PDHB Monoclonal antibody, PBS Only

PDHB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A7C10

Cat no : 68238-1-PBS

Print datasheet

Synonyms

PHE1B, PDHE1-B, PDHE1B, EC:1.2.4.1, 2A7C10

验证数据展示

  • WB analysis using 68238-1-Ig (same clone as 68238-1-PBS)

    WB analysis using 68238-1-Ig (same clone as 68238-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68238-1-Ig (PDHB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 68238-1-Ig (same clone as 68238-1-PBS)

    IHC staining of mouse kidney using 68238-1-Ig (same clone as 68238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDHB antibody included
  • IF Staining of HeLa using 68238-1-Ig (same clone as 68238-1-PBS)

    IF Staining of HeLa using 68238-1-Ig (same clone as 68238-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDHB antibody (68238-1-Ig, Clone: 2A7C10 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68238-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PDHB Ready-To-Use IHC Kit. PDHB primary antibody included.

产品信息

68238-1-PBS targets PDHB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen PDHB fusion protein Ag6857 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name pyruvate dehydrogenase (lipoamide) beta
Synonyms PHE1B, PDHE1-B, PDHE1B, EC:1.2.4.1, 2A7C10
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC000439
Gene Symbol PDHB
Gene ID (NCBI) 5162
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP11177
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDHB (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial) is also named PHE1B. The enzyme, which is found in mitochondria, is one of the component enzymes of the pyruvate dehydrogenase multienzyme complex (PDH). It catalyzes the first reaction of an oxidative decarboxylation sequence converting pyruvate to acetyl-CoA and CO(2)(PMID:3422424). Defects in PDHB are the cause of pyruvate dehydrogenase E1-beta deficiency (PDHBD)(PMID:15138885).