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PDI Recombinant antibody

PDI Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4K17

Cat no : 80170-1-RR

Print datasheet

Synonyms

P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1

验证数据展示

  • WB analysis using 80170-1-RR

    WB analysis using 80170-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80170-1-RR (PDI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 80170-1-RR

    IHC staining of human prostate cancer using 80170-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80170-1-RR (PDI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDI/P4HB antibody included
  • Affinity and Kinetic Characterization of 80170-1-RR

    Affinity and Kinetic Characterization of 80170-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80170-1-RR against Human PDI were performed. The affinity constant is 13.8 nM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman prostate cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PDI/P4HB Ready-To-Use IHC Kit. PDI/P4HB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

80170-1-RR targets PDI in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen PDI fusion protein Ag1747 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name prolyl 4-hydroxylase, beta polypeptide
Synonyms P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC014504
Gene Symbol PDI
Gene ID (NCBI) 5034
RRIDAB_2918871
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07237
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDI antibody 80170-1-RRDownload protocol
IHC protocol for PDI antibody 80170-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Cancer Prev Res (Phila)

Preventive Treatment with PD-1 Antibody Increases Tissue-Resident Memory T Cells Infiltration and Delays Esophageal Carcinogenesis

Authors - Zeru Xiao
View Article