验证数据展示
经过测试的应用
| Positive WB detected in | HSC-T6 cells, PC-12 cells, HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PDI/P4HB Ready-To-Use IHC Kit. PDI/P4HB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80170-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-80170 targets PDI in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | PDI fusion protein Ag1747 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | prolyl 4-hydroxylase, beta polypeptide |
| Synonyms | P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC014504 |
| Gene Symbol | PDI |
| Gene ID (NCBI) | 5034 |
| RRID | AB_3673722 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P07237 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 PDI antibody CL750-80170 | Download protocol |
| FC protocol for CL Plus 750 PDI antibody CL750-80170 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |