CoraLite® Plus 750-conjugated PDI Recombinant antibody

PDI Uni-rAbTM Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-80170

产品说明书

CloneNo. 4K17

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra)

P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格: 

-/ -


经过测试的应用

Positive WB detected inHSC-T6 cells, PC-12 cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PDI/P4HB Ready-To-Use IHC Kit. PDI/P4HB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80170-1-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-80170 targets PDI in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 PDI fusion protein Ag1747 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 prolyl 4-hydroxylase, beta polypeptide
别名 P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1
计算分子量 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC014504
基因名称 PDI
Gene ID (NCBI) 5034
RRIDAB_3673722
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07237
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 PDI antibody CL750-80170Download protocol
FC protocol for CL Plus 750 PDI antibody CL750-80170Download protocol
Standard Protocols
Click here to view our Standard Protocols
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