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CoraLite® Plus 750-conjugated PDI Recombinant antibody

PDI Uni-rAbTM Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

4K17

Cat no : CL750-80170

Print datasheet

Synonyms

P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1

验证数据展示

  • WB analysis using CL750-80170

    WB analysis using CL750-80170

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-80170 (PDI antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using CL750-80170

    FC experiment of HeLa using CL750-80170

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated PDI Recombinant antibody (CL750-80170, Clone:4K17)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHSC-T6 cells, PC-12 cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PDI/P4HB Ready-To-Use IHC Kit. PDI/P4HB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80170-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-80170 targets PDI in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen PDI fusion protein Ag1747 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name prolyl 4-hydroxylase, beta polypeptide
Synonyms P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC014504
Gene Symbol PDI
Gene ID (NCBI) 5034
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07237
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 PDI antibody CL750-80170Download protocol
FC protocol for CL Plus 750 PDI antibody CL750-80170Download protocol
Standard Protocols
Click here to view our Standard Protocols