CoraLite®594-conjugated PDI Monoclonal antibody
PDI Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2E6A11
验证数据展示
经过测试的应用
Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy PDI/P4HB Ready-To-Use IHC Kit. PDI/P4HB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66422-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66422 targets PDI in IF applications and shows reactivity with human, pig, mouse, rat samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, pig, mouse, rat |
Immunogen | PDI fusion protein Ag1747 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | prolyl 4-hydroxylase, beta polypeptide |
Synonyms | Cell and organelle markers, DSI, endoplasmic reticulum marker, ER marker, ERBA2L, GIT, Mitochondria Marker, Mitochondrial Marker, P4HB, P4Hbeta, p55, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, Protein disulfide isomerase |
Calculated Molecular Weight | 57 kDa |
GenBank Accession Number | BC014504 |
Gene Symbol | P4HB |
Gene ID (NCBI) | 5034 |
RRID | AB_2883581 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P07237 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).
实验方案
Product Specific Protocols | |
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IF protocol for CL594 PDI antibody CL594-66422 | Download protocol |
FC protocol for CL594 PDI antibody CL594-66422 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |