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PDIA6 Monoclonal antibody, PBS Only

PDIA6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G2D4

Cat no : 66669-1-PBS

Print datasheet

Synonyms

ER protein 5, ERP5, P5, PDIA6, Protein disulfide isomerase A6, Protein disulfide isomerase P5, TXNDC7

验证数据展示

  • WB analysis using 66669-1-Ig (same clone as 66669-1-PBS)

    WB analysis using 66669-1-Ig (same clone as 66669-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66669-1-Ig (PDIA6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66669-1-Ig (same clone as 66669-1-PBS)

    IF Staining of HepG2 using 66669-1-Ig (same clone as 66669-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PDIA6 antibody (66669-1-Ig, Clone: 1G2D4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

    IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDIA6 antibody included
  • IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

    IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDIA6 antibody included
  • IF Staining of HepG2 using 66669-1-Ig (same clone as 66669-1-PBS)

    IF Staining of HepG2 using 66669-1-Ig (same clone as 66669-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PDIA6 antibody (66669-1-Ig, Clone: 1G2D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PDIA6 Ready-To-Use IHC Kit. PDIA6 primary antibody included.

产品信息

66669-1-PBS targets PDIA6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen PDIA6 fusion protein Ag27608 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein disulfide isomerase family A, member 6
Synonyms ER protein 5, ERP5, P5, PDIA6, Protein disulfide isomerase A6, Protein disulfide isomerase P5, TXNDC7
Calculated Molecular Weight 48 kDa, 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC001312
Gene Symbol PDIA6
Gene ID (NCBI) 10130
RRIDAB_2882023
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15084
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDIA6 (Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family. It is one of the endoplasmic reticulum (ER) resident proteins that catalyze the formation, reduction, and isomerization of disulfide bonds in proteins and is thought to play a role in the folding of disulfide-bonded proteins (PMID:7590364). It has 2 isoforms produced by alternative splicing. This antibody is specific to PDIA6.