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PDIA6 Monoclonal antibody

PDIA6 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G2D4

Cat No : 66669-1-Ig

Print datasheet

Synonyms

ER protein 5, ERP5, P5, PDIA6, Protein disulfide isomerase A6, Protein disulfide isomerase P5, TXNDC7

验证数据展示

  • WB analysis using 66669-1-Ig

    WB analysis using 66669-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66669-1-Ig (PDIA6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HepG2 using 66669-1-Ig

    IF Staining of HepG2 using 66669-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66669-1-Ig (PDIA6 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human liver cancer using 66669-1-Ig

    IHC staining of human liver cancer using 66669-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 10x lens).

    Ready-to-use IHC kits available with PDIA6 antibody included
  • IHC staining of human liver cancer using 66669-1-Ig

    IHC staining of human liver cancer using 66669-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 40x lens).

    Ready-to-use IHC kits available with PDIA6 antibody included
  • IF Staining of HepG2 using 66669-1-Ig

    IF Staining of HepG2 using 66669-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PDIA6 antibody (66669-1-Ig, Clone: 1G2D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PDIA6 Ready-To-Use IHC Kit. PDIA6 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66669-1-Ig targets PDIA6 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Mouse, Rat
Cited Reactivityzebrafish
Immunogen PDIA6 fusion protein Ag27608 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein disulfide isomerase family A, member 6
Synonyms ER protein 5, ERP5, P5, PDIA6, Protein disulfide isomerase A6, Protein disulfide isomerase P5, TXNDC7
Calculated Molecular Weight 48 kDa, 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC001312
Gene Symbol PDIA6
Gene ID (NCBI) 10130
RRIDAB_2882023
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15084
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDIA6 (Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family. It is one of the endoplasmic reticulum (ER) resident proteins that catalyze the formation, reduction, and isomerization of disulfide bonds in proteins and is thought to play a role in the folding of disulfide-bonded proteins (PMID:7590364). It has 2 isoforms produced by alternative splicing. This antibody is specific to PDIA6.

实验方案

Product Specific Protocols
WB protocol for PDIA6 antibody 66669-1-IgDownload protocol
IHC protocol for PDIA6 antibody 66669-1-IgDownload protocol
IF protocol for PDIA6 antibody 66669-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
zebrafishWB

Environ Pollut

Chemical and biological transfer: Which one is responsible for the maternal transfer toxicity of tris(1,3-dichloro-2-propyl) phosphate in zebrafish?

Authors - Yongkang Zhang
View Article