• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HepG2 using CL594-66669

    IF Staining of HepG2 using CL594-66669

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66669 (PDIA6 antibody) at dilution of 1:100.

  • FC experiment of HepG2 using CL594-66669

    FC experiment of HepG2 using CL594-66669

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PDIA6 (CL594-66669, Clone:1G2D4) (red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated PDIA6 Monoclonal antibody

PDIA6 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66669

产品说明书

CloneNo. 1G2D4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Protein disulfide isomerase P5, Protein disulfide isomerase A6, P5, ERp5, ER protein 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PDIA6 Ready-To-Use IHC Kit. PDIA6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66669-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66669 targets PDIA6 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 PDIA6 fusion protein Ag27608 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein disulfide isomerase family A, member 6
别名 Protein disulfide isomerase P5, Protein disulfide isomerase A6, P5, ERp5, ER protein 5
计算分子量 48 kDa, 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC001312
基因名称 PDIA6
Gene ID (NCBI) 10130
RRIDAB_2883612
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15084
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDIA6(Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family. It is one of the endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins (PMID:7590364). It has 2 isoforms produced by alternative splicing. This antibody is specific to PDIA6.

实验方案

Product Specific Protocols
IF protocol for CL594 PDIA6 antibody CL594-66669Download protocol
Standard Protocols
Click here to view our Standard Protocols