验证数据展示
经过测试的应用
Positive IF/ICC detected in | U2OS cells |
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-84117 targets PDLIM4 in IF/ICC applications and shows reactivity with human samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human |
Immunogen | PDLIM4 fusion protein Ag13424 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | PDZ and LIM domain 4 |
Synonyms | |
Calculated Molecular Weight | 35 kDa |
Observed Molecular Weight | 35 kDa |
GenBank Accession Number | BC016765 |
Gene Symbol | PDLIM4 |
Gene ID (NCBI) | 8572 |
RRID | AB_3673342 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P50479 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDLIM4, also known as RIL, is a member of the ALP/Enigma family. PDLIM4 contains one PDZ(DHR) domain and one LIM zinc-binding domain. The LIM zinc-binding domain interacts with the second and fourth PDZ domains of Fap-1, possibly resulting in the dephosphorylation of PDLIM4 (PMID: 21636573). PDLIM4 is involved in osteoblast development and function. The variation of the PDLIM4 locus may be an important determinant of osteoporosis (PMID:12908099).
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 PDLIM4 antibody CL488-84117 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |