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验证数据展示

  • WB analysis using 67651-1-Ig

    WB analysis using 67651-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67651-1-Ig (PDLIM5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

PDLIM5 Monoclonal antibody

PDLIM5 Monoclonal Antibody for WB, ELISA
Cat No. 67651-1-Ig

产品说明书

CloneNo. 1H9C9

宿主/亚型

Mouse / IgM

种属反应性

Human

应用

WB, ELISA

别名

ENH, ENH1, Enigma homolog, L9, LIM, PDLIM5, PDZ and LIM domain 5, PDZ and LIM domain protein 5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue, human platelets

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67651-1-Ig targets PDLIM5 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 PDLIM5 fusion protein Ag25059 种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 PDZ and LIM domain 5
别名 ENH, ENH1, Enigma homolog, L9, LIM, PDLIM5, PDZ and LIM domain 5, PDZ and LIM domain protein 5
计算分子量 64 kDa
观测分子量 63-69 kDa
GenBank蛋白编号BC008741
基因名称 PDLIM5
Gene ID (NCBI) 10611
RRIDAB_2882850
偶联类型 Unconjugated
形式 Liquid
纯化方式Euglobulin precipitation
UNIPROT IDQ96HC4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDLIM5 (PDZ and LIM domain 5), formerly known as enigma homolog (ENH), is a cytoplasmic protein containing PDZ domain and LIM domain at N terminus and C terminus, respectively. PDLIM5 gene generates several splicing variants, EZH1 to EZH4. The long isoform, ENH1, is widely expressed in various tissues including heart, brain, spleen, liver and kidney. In comparison, shorter isoforms that lack the LIM motifs are expressed in cardiac (ENH3) and skeletal muscle (ENH2, ENH3, and ENH4). ENH1 is essential for differentiation of striated muscles through interaction with other proteins. In addition, PDLIM5 is also involved in mood disorders, such as schizophrenia, bipolar disorder, and major depression.

实验方案

Product Specific Protocols
WB protocol for PDLIM5 antibody 67651-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols