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PEA15 Recombinant antibody

PEA15 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241044H6

Cat no : 83958-3-RR

Print datasheet

Synonyms

MAT1H, MAT1, HUMMAT1H, HMAT1, Astrocytic phosphoprotein PEA-15

验证数据展示

  • IF Staining of A431 using 83958-3-RR

    IF Staining of A431 using 83958-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PEA15 antibody (83958-3-RR, Clone: 241044H6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of A431 using 83958-3-RR

    IF Staining of A431 using 83958-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PEA15 antibody (83958-3-RR, Clone: 241044H6 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • Affinity and Kinetic Characterization of 83958-3-RR

    Affinity and Kinetic Characterization of 83958-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83958-3-RR against Human PEA15 were performed. The affinity constant is 6.95 nM.

经过测试的应用

Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83958-3-RR targets PEA15 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen PEA15 fusion protein Ag13782 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phosphoprotein enriched in astrocytes 15
Synonyms MAT1H, MAT1, HUMMAT1H, HMAT1, Astrocytic phosphoprotein PEA-15
Calculated Molecular Weight 130 aa, 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC002426
Gene Symbol PEA15
Gene ID (NCBI) 8682
RRIDAB_3671536
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ15121
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phosphoprotein Enriched in Astrocytes 15 kDa (PEA15), also known as PED-15, is a small, death effector-domain (DED)-containing protein that was recently demonstrated to inhibit tumor necrosis factor-α-induced apoptosis and to reverse the inhibition of integrin activation due to H-Ras. PEA15 is a 15 kDa protein that is highly expressed in the nervous system with particularly high levels in astrocytes and neurons of the hippocampus. PEA15 is a cytoplasmic protein that sits at an important junction in intracellular signalling and can regulate diverse cellular processes, such as proliferation and apoptosis, dependent upon stimulation.

实验方案

Product Specific Protocols
IF protocol for PEA15 antibody 83958-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols