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PEF1 Polyclonal antibody

PEF1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10151-1-AP

Print datasheet

Synonyms

ABP32, PEF1, PEF1A, PEFLIN

验证数据展示

  • WB analysis using 10151-1-AP

    WB analysis using 10151-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10151-1-AP (PEF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 10151-1-AP

    IP experiment of HEK-293 using 10151-1-AP

    IP result of anti-PEF1 (IP:10151-1-AP, 4ug; Detection:10151-1-AP 1:500) with HEK-293 cells lysate 3200ug.

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, HeLa cells
Positive IP detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10151-1-AP targets PEF1 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen PEF1 fusion protein Ag0198 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name penta-EF-hand domain containing 1
Synonyms ABP32, PEF1, PEF1A, PEFLIN
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC002773
Gene Symbol PEF1
Gene ID (NCBI) 553115
RRIDAB_2161168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBV8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PEF1 antibody 10151-1-APDownload protocol
IP protocol for PEF1 antibody 10151-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

J Proteome Res

Proteomic dissection of cell type-specific H2AX-interacting protein complex associated with hepatocellular carcinoma.

Authors - Yang Xiaoli X
View Article