• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PEG3 Recombinant antibody

PEG3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241234C5

Cat no : 84030-5-RR

Print datasheet

Synonyms

Zinc finger and SCAN domain-containing protein 24, PW1, Paternally-expressed gene 3 protein, paternally expressed 3, KIAA0287

验证数据展示

  • WB analysis of TT using 84030-5-RR

    WB analysis of TT using 84030-5-RR

    TT cells were subjected to SDS PAGE followed by western blot with 84030-5-RR (PEG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 84030-5-RR

    IF Staining of HepG2 using 84030-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PEG3 antibody (84030-5-RR, Clone: 241234C5 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 84030-5-RR

    FC experiment of HepG2 using 84030-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PEG3 Recombinant antibody (84030-5-RR, Clone:241234C5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • WB analysis of HepG2 using 84030-5-RR

    WB analysis of HepG2 using 84030-5-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 84030-5-RR (PEG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 84030-5-RR

    IHC staining of mouse brain using 84030-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84030-5-RR (PEG3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 84030-5-RR

    IHC staining of mouse brain using 84030-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84030-5-RR (PEG3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84030-5-RR

    Affinity and Kinetic Characterization of 84030-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84030-5-RR against Human PEG3 were performed. The affinity constant is 52.3 pM.

经过测试的应用

Positive WB detected inTT cells, HepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84030-5-RR targets PEG3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen PEG3 fusion protein Ag20132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name paternally expressed 3
Synonyms Zinc finger and SCAN domain-containing protein 24, PW1, Paternally-expressed gene 3 protein, paternally expressed 3, KIAA0287
Calculated Molecular Weight 1588 aa, 181 kDa
GenBank Accession NumberBC150272
Gene Symbol PEG3
Gene ID (NCBI) 5178
RRIDAB_3671596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9GZU2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Paternally expressed 3 (PEG3) was responsible for Kras/Erk- and mTOR-driven HCC (hepatocellular carcinoma) (PMID: 36451866). PEG3 can regulate the physiological processes related to energy homeostasis found in multiple tissues, such as the brain, testis, ovary, and placenta (PMID: 32729618). Epigenetic silencing and loss of imprinting of PEG3 are implicated in glioma tumorigenesis (PMID: 32231463).

实验方案

Product Specific Protocols
WB protocol for PEG3 antibody 84030-5-RRDownload protocol
IHC protocol for PEG3 antibody 84030-5-RRDownload protocol
IF protocol for PEG3 antibody 84030-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols