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CoraLite® Plus 488-conjugated PEG3 Recombinant antibody

PEG3 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241234C5

Cat no : CL488-84030-5

Print datasheet

Synonyms

Zinc finger and SCAN domain-containing protein 24, PW1, Paternally-expressed gene 3 protein, paternally expressed 3, KIAA0287

验证数据展示

  • FC experiment of HepG2 using CL488-84030-5

    FC experiment of HepG2 using CL488-84030-5

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Peg3 Recombinant Antibody (CL488-84030-5, Clone:241234C5)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 84030-5-PBS

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84030-5 targets PEG3 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen PEG3 fusion protein Ag20132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name paternally expressed 3
Synonyms Zinc finger and SCAN domain-containing protein 24, PW1, Paternally-expressed gene 3 protein, paternally expressed 3, KIAA0287
Calculated Molecular Weight 1588 aa, 181 kDa
GenBank Accession NumberBC150272
Gene Symbol PEG3
Gene ID (NCBI) 5178
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9GZU2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Paternally expressed 3 (PEG3) was responsible for Kras/Erk- and mTOR-driven HCC (hepatocellular carcinoma) (PMID: 36451866). PEG3 can regulate the physiological processes related to energy homeostasis found in multiple tissues, such as the brain, testis, ovary, and placenta (PMID: 32729618). Epigenetic silencing and loss of imprinting of PEG3 are implicated in glioma tumorigenesis (PMID: 32231463).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PEG3 antibody CL488-84030-5Download protocol
Standard Protocols
Click here to view our Standard Protocols