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验证数据展示

  • FC experiment of HepG2 using CL488-84030-5

    FC experiment of HepG2 using CL488-84030-5

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Peg3 Recombinant Antibody (CL488-84030-5, Clone:241234C5)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated PEG3 Recombinant antibody

PEG3 Recombinant Antibody for FC (Intra)
Cat No. CL488-84030-5

产品说明书

CloneNo. 241234C5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

Zinc finger and SCAN domain-containing protein 24, PW1, Paternally-expressed gene 3 protein, paternally expressed 3, KIAA0287

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 84030-5-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84030-5 targets PEG3 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 PEG3 fusion protein Ag20132 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 paternally expressed 3
别名 Zinc finger and SCAN domain-containing protein 24, PW1, Paternally-expressed gene 3 protein, paternally expressed 3, KIAA0287
计算分子量 1588 aa, 181 kDa
GenBank蛋白编号BC150272
基因名称 PEG3
Gene ID (NCBI) 5178
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9GZU2
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Paternally expressed 3 (PEG3) was responsible for Kras/Erk- and mTOR-driven HCC (hepatocellular carcinoma) (PMID: 36451866). PEG3 can regulate the physiological processes related to energy homeostasis found in multiple tissues, such as the brain, testis, ovary, and placenta (PMID: 32729618). Epigenetic silencing and loss of imprinting of PEG3 are implicated in glioma tumorigenesis (PMID: 32231463).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PEG3 antibody CL488-84030-5Download protocol
Standard Protocols
Click here to view our Standard Protocols