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验证数据展示

  • WB analysis of SH-SY5Y using 85099-1-RR (same clone as 85099-1-PBS)

    WB analysis of SH-SY5Y using 85099-1-RR (same clone as 85099-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 85099-1-RR (PEMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

    IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

    IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85099-1-RR

    Affinity and Kinetic Characterization of 85099-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85099-1-RR against Human PEMT were performed. The affinity constant is 0.379 nM.

PEMT Recombinant monoclonal antibody, PBS Only

PEMT Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85099-1-PBS

产品说明书

CloneNo. 242150D5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

Phosphatidylethanolamine N-methyltransferase, PEMT2, PEMPT, PEAMT, EC:2.1.1.17

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85099-1-PBS targets PEMT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8034

Product name: Recombinant human PEMT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-236 aa of BC050593

Sequence: MKRSGNPGAEVTNSSVAGPDCCGGLGNIDFRQADFCVMTRLLGYVDPLDPSFVAAVITITFNPLYWNVVARWEHKTRKLSRAFGSPYLACYSLSVTILLLNFLRSHCFTQAMLSQPRMESLDTPAAYSLGLALLGLGVVLVLSSFFALGFAGTFLGDYFGILKEARVTVFPFNILDNPMYWGSTANYLGWAIMHASPTGLLLTVLVALTYIVALLYEEPFTAEIYRQKASGSHKRS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphatidylethanolamine N-methyltransferase
别名 Phosphatidylethanolamine N-methyltransferase, PEMT2, PEMPT, PEAMT, EC:2.1.1.17
计算分子量 26 kDa
观测分子量 22-25 kDa
GenBank蛋白编号BC050593
基因名称 PEMT
Gene ID (NCBI) 10400
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UBM1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PEMT Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure (PMID:12431977, 15927961).

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