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PEMT Recombinant antibody, PBS Only

PEMT Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242150D5

Cat no : 85099-1-PBS

Print datasheet

Synonyms

Phosphatidylethanolamine N-methyltransferase, PEMT2, PEMPT, PEAMT, EC:2.1.1.17

验证数据展示

  • WB analysis of SH-SY5Y using 85099-1-RR (same clone as 85099-1-PBS)

    WB analysis of SH-SY5Y using 85099-1-RR (same clone as 85099-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 85099-1-RR (PEMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

    IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

    IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

产品信息

85099-1-PBS targets PEMT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen PEMT fusion protein Ag8034 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phosphatidylethanolamine N-methyltransferase
Synonyms Phosphatidylethanolamine N-methyltransferase, PEMT2, PEMPT, PEAMT, EC:2.1.1.17
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC050593
Gene Symbol PEMT
Gene ID (NCBI) 10400
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UBM1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PEMT Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure (PMID:12431977, 15927961).