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验证数据展示

  • WB analysis of SH-SY5Y using 85099-1-RR

    WB analysis of SH-SY5Y using 85099-1-RR

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 85099-1-RR (PEMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 85099-1-RR

    IHC staining of mouse liver using 85099-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 85099-1-RR

    IHC staining of mouse liver using 85099-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85099-1-RR

    Affinity and Kinetic Characterization of 85099-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85099-1-RR against Human PEMT were performed. The affinity constant is 0.379 nM.

PEMT Recombinant antibody

PEMT Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85099-1-RR

产品说明书

CloneNo. 242150D5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Phosphatidylethanolamine N-methyltransferase, PEMT2, PEMPT, PEAMT, EC:2.1.1.17

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85099-1-RR targets PEMT in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PEMT fusion protein Ag8034 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphatidylethanolamine N-methyltransferase
别名 Phosphatidylethanolamine N-methyltransferase, PEMT2, PEMPT, PEAMT, EC:2.1.1.17
计算分子量 26 kDa
观测分子量 22-25 kDa
GenBank蛋白编号BC050593
基因名称 PEMT
Gene ID (NCBI) 10400
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UBM1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PEMT Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure (PMID:12431977, 15927961).

实验方案

Product Specific Protocols
WB protocol for PEMT antibody 85099-1-RRDownload protocol
IHC protocol for PEMT antibody 85099-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols