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PEMT Recombinant antibody

PEMT Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

242150D5

Cat no : 85099-1-RR

Print datasheet

Synonyms

Phosphatidylethanolamine N-methyltransferase, PEMT2, PEMPT, PEAMT, EC:2.1.1.17

验证数据展示

  • WB analysis of SH-SY5Y using 85099-1-RR

    WB analysis of SH-SY5Y using 85099-1-RR

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 85099-1-RR (PEMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 85099-1-RR

    IHC staining of mouse liver using 85099-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 85099-1-RR

    IHC staining of mouse liver using 85099-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85099-1-RR targets PEMT in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PEMT fusion protein Ag8034 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phosphatidylethanolamine N-methyltransferase
Synonyms Phosphatidylethanolamine N-methyltransferase, PEMT2, PEMPT, PEAMT, EC:2.1.1.17
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC050593
Gene Symbol PEMT
Gene ID (NCBI) 10400
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UBM1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PEMT Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure (PMID:12431977, 15927961).

实验方案

Product Specific Protocols
WB protocol for PEMT antibody 85099-1-RRDownload protocol
IHC protocol for PEMT antibody 85099-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols