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PEPD Polyclonal antibody

PEPD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12218-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

X-Pro dipeptidase, Xaa-Pro dipeptidase, PROLIDASE, PRD, EC:3.4.13.9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, MDA-MB-453s cells, mouse skin tissue, HeLa cells, HepG2 cells, SH-SY5Y cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:100-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12218-1-AP targets PEPD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 PEPD fusion protein Ag2842 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidase D
别名 X-Pro dipeptidase, Xaa-Pro dipeptidase, PROLIDASE, PRD, EC:3.4.13.9
计算分子量 493 aa, 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC015027
基因名称 PEPD
Gene ID (NCBI) 5184
RRIDAB_2161517
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12955
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PEPD, also named as PRD, Prolidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D and Proline dipeptidase, belongs to the peptidase M24B family and Eukaryotic-type prolidase subfamily. PEPD splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). PEPD is considered as the most promising candidate genes for altering AAA risk, based on gene function, association evidence, gene expression, and protein expression.(PMID:21247474)

实验方案

Product Specific Protocols
WB protocol for PEPD antibody 12218-1-APDownload protocol
IHC protocol for PEPD antibody 12218-1-APDownload protocol
IF protocol for PEPD antibody 12218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

BMC Med Genet

Analysis of positional candidate genes in the AAA1 susceptibility locus for abdominal aortic aneurysms on chromosome 19.

Authors - Lillvis John H JH
humanIHC

Arch Med Res

Relationship Between Echocardiographically Evaluated Aortic Stiffness and Prolidase Activity in Aortic Tissue of Patients with Critical Coronary Artery Disease.

Authors - Aydemir Koçarslan
humanWB

Nat Commun

Parental experiences orchestrate locust egg hatching synchrony by regulating nuclear export of precursor miRNA

Authors - Ya Nan Zhu
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