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  • Featured Product
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PEPD Polyclonal antibody

PEPD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 12218-1-AP

Print datasheet

Synonyms

X-Pro dipeptidase, Xaa-Pro dipeptidase, PROLIDASE, PRD, EC:3.4.13.9

验证数据展示

  • WB analysis using 12218-1-AP

    WB analysis using 12218-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 12218-1-AP

    WB analysis of HEK-293 using 12218-1-AP

    WB result of PEPD antibody (12218-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEPD transfected HEK-293 cells.

  • IHC staining of human lung cancer using 12218-1-AP

    IHC staining of human lung cancer using 12218-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12218-1-AP (PEPD antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HepG2 using 12218-1-AP

    IF Staining of HepG2 using 12218-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12218-1-AP (PEPD antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, MDA-MB-453s cells, mouse skin tissue, HeLa cells, HepG2 cells, SH-SY5Y cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:100-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

12218-1-AP targets PEPD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen PEPD fusion protein Ag2842 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptidase D
Synonyms X-Pro dipeptidase, Xaa-Pro dipeptidase, PROLIDASE, PRD, EC:3.4.13.9
Calculated Molecular Weight 493 aa, 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC015027
Gene Symbol PEPD
Gene ID (NCBI) 5184
RRIDAB_2161517
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12955
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PEPD, also named as PRD, Prolidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D and Proline dipeptidase, belongs to the peptidase M24B family and Eukaryotic-type prolidase subfamily. PEPD splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). PEPD is considered as the most promising candidate genes for altering AAA risk, based on gene function, association evidence, gene expression, and protein expression.(PMID:21247474)

实验方案

Product Specific Protocols
WB protocol for PEPD antibody 12218-1-APDownload protocol
IHC protocol for PEPD antibody 12218-1-APDownload protocol
IF protocol for PEPD antibody 12218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

BMC Med Genet

Analysis of positional candidate genes in the AAA1 susceptibility locus for abdominal aortic aneurysms on chromosome 19.

Authors - Lillvis John H JH
View Article
humanIHC

Arch Med Res

Relationship Between Echocardiographically Evaluated Aortic Stiffness and Prolidase Activity in Aortic Tissue of Patients with Critical Coronary Artery Disease.

Authors - Aydemir Koçarslan
View Article