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CoraLite® Plus 488-conjugated PER2 (isoform 1) Monoclonal antibody

PER2 (isoform 1) Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E6C3

Cat no : CL488-67513

Print datasheet

Synonyms

PER2, PER 2, hPER2, FASPS, 2E6C3

验证数据展示

  • IF Staining of HepG2 using CL488-67513

    IF Staining of HepG2 using CL488-67513

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PER2 (isoform 1) antibody (CL488-67513, Clone: 2E6C3 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-67513

    FC experiment of HeLa using CL488-67513

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Per2 (Isoform 1) Monoclonal Antibody (CL488-67513, Clone:2E6C3)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PER2 Ready-To-Use IHC Kit. PER2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67513-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67513 targets PER2 (isoform 1) in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen PER2 (isoform 1) fusion protein Ag29489 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name period homolog 2 (Drosophila)
Synonyms PER2, PER 2, hPER2, FASPS, 2E6C3
Calculated Molecular Weight 137 kDa
Observed Molecular Weight150~160 kDa
GenBank Accession NumberNM_022817
Gene Symbol PER2
Gene ID (NCBI) 8864
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO15055
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. There are two isoforms of PER2: isoform 1 has an expected molecular size of about 140 kDa, and 67513-1-Ig detected molecular weight of 150-160 kDa (PMID:16675517), while isoform 2, known as PER2S (a second splicing variant of the human PER2 gene), has a molecular weight of about 45 kDa (PMID:26347774).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PER2 (isoform 1) antibody CL488-67513Download protocol
FC protocol for CL Plus 488 PER2 (isoform 1) antibody CL488-67513Download protocol
Standard Protocols
Click here to view our Standard Protocols