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验证数据展示

  • WB analysis using 10594-1-AP

    WB analysis using 10594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10594-1-AP (PEX14 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 10594-1-AP

    IHC staining of mouse liver using 10594-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10594-1-AP (PEX14 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 10594-1-AP

    IHC staining of mouse liver using 10594-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10594-1-AP (PEX14 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 10594-1-AP

    IF Staining of HeLa using 10594-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PEX14 antibody (10594-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • WB analysis of HEK-293 using 10594-1-AP

    WB analysis of HEK-293 using 10594-1-AP

    WB result of PEX14 antibody (10594-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEX14 transfected HEK-293 cells.

  • IP experiment of mouse liver using 10594-1-AP

    IP experiment of mouse liver using 10594-1-AP

    IP result of anti-PEX14 (IP:10594-1-AP, 3ug; Detection:10594-1-AP 1:500) with mouse liver tissue lysate 4000ug.

  • FC experiment of HeLa using 10594-1-AP

    FC experiment of HeLa using 10594-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PEX14 (10594-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

PEX14 Polyclonal antibody

PEX14 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10594-1-AP

产品说明书

引用文献(63)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey and More (2)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

别名

PTS1 receptor docking protein, Pex14p, PEX, Peroxin-14, Peroxin 14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HeLa cells
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse liver tissue, human liver cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, COS7 cells, HepG2 cells, NIH/3T3 cells, C2C12 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10594-1-AP targets PEX14 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat, monkey
文献引用反应性human, mouse, rat, monkey, chicken, yeast
免疫原 PEX14 fusion protein Ag0932 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peroxisomal biogenesis factor 14
别名 PTS1 receptor docking protein, Pex14p, PEX, Peroxin-14, Peroxin 14
计算分子量 41 kDa
观测分子量 57 kDa
GenBank蛋白编号BC006327
基因名称 PEX14
Gene ID (NCBI) 5195
RRIDAB_2252194
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75381
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

实验方案

Product Specific Protocols
WB protocol for PEX14 antibody 10594-1-APDownload protocol
IHC protocol for PEX14 antibody 10594-1-APDownload protocol
IF protocol for PEX14 antibody 10594-1-APDownload protocol
IP protocol for PEX14 antibody 10594-1-APDownload protocol
FC protocol for PEX14 antibody 10594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Science

Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

Authors - Shun Nagashima
View Article
mouseIF

Cell

A Network of Macrophages Supports Mitochondrial Homeostasis in the Heart.

Authors - José A Nicolás-Ávila
View Article
mouseIF

Nat Cell Biol

Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties

Authors - Dennis K Jeppesen
View Article
humanIF

Mol Cell

The HRI branch of the integrated stress response selectively triggers mitophagy

Authors - Yogaditya Chakrabarty
View Article
mouseIF

Mol Cell

Drp1 Tubulates the ER in a GTPase-Independent Manner.

Authors - Yoshihiro Adachi
View Article
mouseWB, IHC

Cell Metab

Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy.

Authors - Katharina M Walter
View Article