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验证数据展示

  • IF Staining of HeLa using CL555-10594

    IF Staining of HeLa using CL555-10594

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®555 PEX14 antibody (CL555-10594) at dilution of 1:200.

  • FC experiment of HeLa using CL555-10594

    FC experiment of HeLa using CL555-10594

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555-conjugated PEX14 Polyclonal antibody (CL555-10594)(red), or 0.4 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®555-conjugated PEX14 Polyclonal antibody

PEX14 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-10594

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey

应用

IF/ICC, FC (Intra)

别名

PTS1 receptor docking protein, Pex14p, PEX, Peroxin-14, Peroxin 14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 10594-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-10594 targets PEX14 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, monkey
免疫原 PEX14 fusion protein Ag0932 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peroxisomal biogenesis factor 14
别名 PTS1 receptor docking protein, Pex14p, PEX, Peroxin-14, Peroxin 14
计算分子量 41 kDa
观测分子量 57 kDa
GenBank蛋白编号BC006327
基因名称 PEX14
Gene ID (NCBI) 5195
RRIDAB_2919590
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75381
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

实验方案

Product Specific Protocols
IF protocol for CL555 PEX14 antibody CL555-10594Download protocol
Standard Protocols
Click here to view our Standard Protocols