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验证数据展示

  • WB analysis using 68555-1-Ig

    WB analysis using 68555-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68555-1-Ig (PEX19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68555-1-Ig

    IF Staining of HeLa using 68555-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PEX19 antibody (68555-1-Ig, Clone: 3H2C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

  • IF Staining of Jurkat using 68555-1-Ig

    IF Staining of Jurkat using 68555-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PEX19 antibody (68555-1-Ig, Clone: 3H2C2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

PEX19 Monoclonal antibody

PEX19 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68555-1-Ig

产品说明书

CloneNo. 3H2C2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IF/ICC, ELISA

别名

PMPI, PMP1, PEX, Peroxin-19, OK/SW-cl.22

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, A549 cells, Jurkat cells, K-562 cells, Pig liver tissue, Rat liver tissue, Mouse liver tissue
Positive IF/ICC detected inJurkat cells, HeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68555-1-Ig targets PEX19 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 PEX19 fusion protein Ag6858 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 peroxisomal biogenesis factor 19
别名 PMPI, PMP1, PEX, Peroxin-19, OK/SW-cl.22
计算分子量 33 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC000496
基因名称 PEX19
Gene ID (NCBI) 5824
RRIDAB_3085258
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP40855
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. PEX19 gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs) (PMID: 14709540). PEX19 may bind newly synthesized PMPs and facilitate their insertion into the peroxisome membrane (PMID: 107044440. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. Defects in this gene are a cause of Zellweger syndrome (ZWS), as well as peroxisome biogenesis disorder complementation group 14 (PBD-CG14) (PMID:20683989) (PMID:10051604).

实验方案

Product Specific Protocols
WB protocol for PEX19 antibody 68555-1-IgDownload protocol
IF protocol for PEX19 antibody 68555-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols