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验证数据展示

  • WB analysis using 68555-1-Ig

    WB analysis using 68555-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68555-1-Ig (PEX19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68555-1-Ig

    IF Staining of HeLa using 68555-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PEX19 antibody (68555-1-Ig, Clone: 3H2C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

  • IF Staining of Jurkat using 68555-1-Ig

    IF Staining of Jurkat using 68555-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PEX19 antibody (68555-1-Ig, Clone: 3H2C2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

PEX19 Monoclonal antibody

PEX19 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68555-1-Ig
CloneNo. 3H2C2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

PMPI, PMP1, PEX, Peroxin-19, OK/SW-cl.22

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, A549 cells, Jurkat cells, K-562 cells, Pig liver tissue, Rat liver tissue, Mouse liver tissue
Positive IF/ICC detected inJurkat cells, HeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68555-1-Ig targets PEX19 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen PEX19 fusion protein Ag6858 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name peroxisomal biogenesis factor 19
Synonyms PMPI, PMP1, PEX, Peroxin-19, OK/SW-cl.22
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC000496
Gene Symbol PEX19
Gene ID (NCBI) 5824
RRIDAB_3085258
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP40855
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. PEX19 gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs) (PMID: 14709540). PEX19 may bind newly synthesized PMPs and facilitate their insertion into the peroxisome membrane (PMID: 107044440. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. Defects in this gene are a cause of Zellweger syndrome (ZWS), as well as peroxisome biogenesis disorder complementation group 14 (PBD-CG14) (PMID:20683989) (PMID:10051604).

实验方案

Product Specific Protocols
WB protocol for PEX19 antibody 68555-1-IgDownload protocol
IF protocol for PEX19 antibody 68555-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols