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PFKFB3 Polyclonal antibody
PFKFB3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
117
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, mouse thymus tissue, mouse heart tissue, A431 cells, HeLa cells, Jurkat cells, mouse spleen tissue |
Positive IP detected in | mouse spleen tissue |
Positive IHC detected in | human liver cancer tissue, human kidney tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13763-1-AP targets PFKFB3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | PFKFB3 fusion protein Ag4744 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 |
Synonyms | IPFK2, Fructose-2,6-bisphosphatase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3, 6-phosphofructo-2-kinase, 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme |
Calculated Molecular Weight | 520 aa, 60 kDa |
Observed Molecular Weight | 58 kDa |
GenBank Accession Number | BC040482 |
Gene Symbol | PFKFB3 |
Gene ID (NCBI) | 5209 |
RRID | AB_2162854 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q16875 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PFKFB3 antibody 13763-1-AP | Download protocol |
IHC protocol for PFKFB3 antibody 13763-1-AP | Download protocol |
IF protocol for PFKFB3 antibody 13763-1-AP | Download protocol |
IP protocol for PFKFB3 antibody 13763-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
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