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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PFKFB3-Specific Polyclonal antibody

PFKFB3-Specific Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13763

Print datasheet

Synonyms

6PF 2 K/Fru 2,6 P2ase 3, FLJ37326, IPFK2, PFK/FBPase 3, PFK2, PFKFB3

验证数据展示

  • IF Staining of HepG2 using CL488-13763

    IF Staining of HepG2 using CL488-13763

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PFKFB3-Specific antibody (CL488-13763) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 13763-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13763 targets PFKFB3-Specific in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen PFKFB3-Specific fusion protein Ag4744 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Synonyms 6PF 2 K/Fru 2,6 P2ase 3, FLJ37326, IPFK2, PFK/FBPase 3, PFK2, PFKFB3
Calculated Molecular Weight 520 aa, 60 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC040482
Gene Symbol PFKFB3
Gene ID (NCBI) 5209
RRIDAB_2919078
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16875
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PFKFB3, also named as NY-REN-56 and iPFK-2, plays a role in glucose metabolism. It synthesis and degradation of fructose 2,6-bisphosphate. Endogenously generated adenosine cooperates with bacterial components to increase PFKFB3 isozyme activity, resulting in greater fructose 2,6-bisphosphate accumulation. PFKFB3 is required for increased growth, metabolic activity and is regulated through active JAK2 and STAT5. This antibody is specific to PFKFB3.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PFKFB3-Specific antibody CL488-13763Download protocol
Standard Protocols
Click here to view our Standard Protocols