验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 13763-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-13763 targets PFKFB3-Specific in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | PFKFB3-Specific fusion protein Ag4744 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 |
| 别名 | 6PF 2 K/Fru 2,6 P2ase 3, FLJ37326, IPFK2, PFK/FBPase 3, PFK2, PFKFB3 |
| 计算分子量 | 520 aa, 60 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC040482 |
| 基因名称 | PFKFB3 |
| Gene ID (NCBI) | 5209 |
| RRID | AB_2919078 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16875 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PFKFB3, also named as NY-REN-56 and iPFK-2, plays a role in glucose metabolism. It synthesis and degradation of fructose 2,6-bisphosphate. Endogenously generated adenosine cooperates with bacterial components to increase PFKFB3 isozyme activity, resulting in greater fructose 2,6-bisphosphate accumulation. PFKFB3 is required for increased growth, metabolic activity and is regulated through active JAK2 and STAT5. This antibody is specific to PFKFB3.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PFKFB3-Specific antibody CL488-13763 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |