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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68129-1-Ig (same clone as 68129-1-PBS)

    WB analysis using 68129-1-Ig (same clone as 68129-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68129-1-Ig (PFKP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68129-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68129-1-Ig (same clone as 68129-1-PBS)

    IF Staining of HeLa using 68129-1-Ig (same clone as 68129-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PFKP antibody (68129-1-Ig, Clone: 1H4D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68129-1-PBS in a different storage buffer formulation.

PFKP Monoclonal antibody, PBS Only

PFKP Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68129-1-PBS

产品说明书

CloneNo. 1H4D4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, rabbit

应用

WB, IF/ICC, Indirect ELISA

别名

1H4D4, 6-phosphofructokinase type C, ATP-dependent 6-phosphofructokinase, platelet type, ATP-PFK, EC:2.7.1.11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PFKP Ready-To-Use IHC Kit. PFKP primary antibody included.

产品信息

68129-1-PBS targets PFKP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原 PFKP fusion protein Ag25206 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphofructokinase, platelet
别名 1H4D4, 6-phosphofructokinase type C, ATP-dependent 6-phosphofructokinase, platelet type, ATP-PFK, EC:2.7.1.11
计算分子量 784 aa, 86 kDa
观测分子量80-86 kDa
GenBank蛋白编号BC029138
基因名称 PFKP
Gene ID (NCBI) 5214
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ01813
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).