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  • Featured Product
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CoraLite® Plus 594-conjugated PGAM1 Monoclonal antibody

PGAM1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

2H2A9

Cat no : CL594-67470

Print datasheet

Synonyms

BPG dependent PGAM 1, PGAM B, PGAM1, PGAMA, Phosphoglycerate mutase 1

验证数据展示

  • IF Staining of HepG2 using CL594-67470

    IF Staining of HepG2 using CL594-67470

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 594 PGAM1 antibody (CL594-67470, Clone: 2H2A9 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67470-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67470 targets PGAM1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen PGAM1 fusion protein Ag9250 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphoglycerate mutase 1 (brain)
Synonyms BPG dependent PGAM 1, PGAM B, PGAM1, PGAMA, Phosphoglycerate mutase 1
Calculated Molecular Weight 254 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC011678
Gene Symbol PGAM1
Gene ID (NCBI) 5223
Conjugate CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths594 nm / 615 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP18669
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PGAM1(phosphoglycerate mutase 1) is also named as PGAMA,PGAM-B and belongs to the phosphoglycerate mutase family. Phosphoglycerate mutase is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The homodimer PGAM1 is expressed mainly in liver, kidney, brain and overexpressed in a variety of human cancers, including breast carcinoma, colorectal cancer, lung cancer, prostate cancer, oral squamous cell carcinoma, esophageal squamous cell carcinomas and also associated with certain virus infection. PGAM1 could be developed as a useful diagnostic biomarker, as well as a potential therapeutic target for hepatocellular carcinoma (PMID:20403181). This antibody may also recognize PGAM2 and PGAM4 due to the high homology.

实验方案

Product Specific Protocols
IF protocol for CL Plus 594 PGAM1 antibody CL594-67470Download protocol
Standard Protocols
Click here to view our Standard Protocols