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验证数据展示

  • IF Staining of HepG2 using CL594-67470

    IF Staining of HepG2 using CL594-67470

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 594 PGAM1 antibody (CL594-67470, Clone: 2H2A9 ) at dilution of 1:200.

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  • KD/KO验证

CoraLite® Plus 594-conjugated PGAM1 Monoclonal antibody

PGAM1 Monoclonal Antibody for IF/ICC
Cat No. CL594-67470

产品说明书

CloneNo. 2H2A9

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

IF/ICC

别名

BPG dependent PGAM 1, PGAM B, PGAM1, PGAMA, Phosphoglycerate mutase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67470-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67470 targets PGAM1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Mouse, Rat
免疫原 PGAM1 fusion protein Ag9250 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoglycerate mutase 1 (brain)
别名 BPG dependent PGAM 1, PGAM B, PGAM1, PGAMA, Phosphoglycerate mutase 1
计算分子量 254 aa, 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC011678
基因名称 PGAM1
Gene ID (NCBI) 5223
RRIDAB_3084769
偶联类型 CoraLite® Plus 594 Fluorescent Dye
最大激发/发射波长594 nm / 615 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP18669
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PGAM1(phosphoglycerate mutase 1) is also named as PGAMA,PGAM-B and belongs to the phosphoglycerate mutase family. Phosphoglycerate mutase is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The homodimer PGAM1 is expressed mainly in liver, kidney, brain and overexpressed in a variety of human cancers, including breast carcinoma, colorectal cancer, lung cancer, prostate cancer, oral squamous cell carcinoma, esophageal squamous cell carcinomas and also associated with certain virus infection. PGAM1 could be developed as a useful diagnostic biomarker, as well as a potential therapeutic target for hepatocellular carcinoma (PMID:20403181). This antibody may also recognize PGAM2 and PGAM4 due to the high homology.

实验方案

Product Specific Protocols
IF protocol for CL Plus 594 PGAM1 antibody CL594-67470Download protocol
Standard Protocols
Click here to view our Standard Protocols