验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, HeLa cells, mouse skeletal muscle tissue, rat heart tisssue, rat skeletal muscle tissue |
| Positive IHC detected in | human breast cancer tissue, mouse skeletal muscle tissue, mouse heart tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15550-1-AP targets PGAM2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 | PGAM2 fusion protein Ag7908 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphoglycerate mutase 2 (muscle) |
| 别名 | BPG dependent PGAM 2, PGAM M, PGAM2, PGAMM, Phosphoglycerate mutase 2 |
| 计算分子量 | 29 kDa |
| 观测分子量 | 29 kDa |
| GenBank蛋白编号 | BC001904 |
| 基因名称 | PGAM2 |
| Gene ID (NCBI) | 5224 |
| RRID | AB_2299336 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P15259 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Phosphoglycerate mutase (PGAM), an important enzyme in the glycolytic pathway, catalyzes the transfer of a phosphate group between the 2 and the 3 positions of glyceric acid. The muscle-specific isoform (type M, PGAM2) of phosphoglycerate mutase (PGAM) is a housekeeping enzyme and it catalyzes the conversion of 3-phosphoglycerate into 2-phosphoglycerate in the glycolysis process to release energy. It is encoded by the Pgam2 gene. In addition, it is demonstrated that PGAM2 locates both in cytoplasm and nuclei, and takes part in the glycometabolism process of cytoplasm and nuclei(PMID: 18499067). Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10). This antibody may also recognize PGAM1 and PGAM4 due to the high homology.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PGAM2 antibody 15550-1-AP | Download protocol |
| IHC protocol for PGAM2 antibody 15550-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Rep METTL14 modulates glycolysis to inhibit colorectal tumorigenesis in p53-wild-type cells | ||
Sci Rep Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs. | ||
J Proteomics Proteomics analysis of asthenozoospermia and identification of glucose-6-phosphate isomerase as an important enzyme for sperm motility. | ||
Redox Biol Unbalanced redox status network as an early pathological event in congenital cataracts | ||
Cell Res Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine synthesis to p53 activation to control cell fate |
