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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated PGC1a Monoclonal antibody

PGC1a Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1C1B2

Cat No : CL647-66369

Print datasheet

Synonyms

LEM6, Ligand effect modulator 6, PGC 1 alpha, PGC 1(alpha), PGC 1v, PGC1, PGC1A, PPAR gamma coactivator 1 alpha, PPARGC 1 alpha, PPARGC1, PPARGC1A

验证数据展示

  • FC experiment of HeLa using CL647-66369

    FC experiment of HeLa using CL647-66369

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human PGC1a (CL647-66369, Clone:1C1B2) (red), or 0.2 ug APC-65128 ; APC Mouse IgG2b Isotype Control (APC-65128, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66369-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66369 targets PGC1a in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
Synonyms LEM6, Ligand effect modulator 6, PGC 1 alpha, PGC 1(alpha), PGC 1v, PGC1, PGC1A, PPAR gamma coactivator 1 alpha, PPARGC 1 alpha, PPARGC1, PPARGC1A
Calculated Molecular Weight 91 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberNM_013261
Gene Symbol PPARGC1A
Gene ID (NCBI) 10891
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UBK2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists an isoform in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 PGC1a antibody CL647-66369Download protocol
Standard Protocols
Click here to view our Standard Protocols