• Featured Product
  • KD/KO Validated

PGC1a Monoclonal antibody

PGC1a Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig and More (6)

Applications

WB, IF/ICC, ELISA and More (5)

Conjugate

Unconjugated

CloneNo.

1C1B2

Cat no : 66369-1-Ig

Print datasheet

Synonyms

PPARGC1A, PGC 1v, PGC 1(alpha), PGC 1 alpha, Ligand effect modulator 6



经过测试的应用

Positive WB detected inpig skeletal muscle tissue, HSC-T6 cells, rat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66369-1-Ig targets PGC1a in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, ChIP, ELISA
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, pig, monkey, chicken, zebrafish, hamster, goat, ducks
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
Synonyms PPARGC1A, PGC 1v, PGC 1(alpha), PGC 1 alpha, Ligand effect modulator 6
Calculated Molecular Weight 91 kDa
Observed Molecular Weight 70 kDa, 100 kDa
GenBank Accession NumberNM_013261
Gene Symbol PGC1a
Gene ID (NCBI) 10891
RRIDAB_2828002
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UBK2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists an isoform in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.

实验方案

Product Specific Protocols
WB protocol for PGC1a antibody 66369-1-IgDownload protocol
IF protocol for PGC1a antibody 66369-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
humanChIP

Adv Sci (Weinh)

Sirtuin1 Suppresses Calcium Oxalate Nephropathy via Inhibition of Renal Proximal Tubular Cell Ferroptosis Through PGC-1α-mediated Transcriptional Coactivation

Authors - Chen Duan
mouseWB

Sci Adv

Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice.

Authors - Jun Seok Son
chickenWB,IF

J Hazard Mater

Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers

Authors - Gaolong Zhong
WB

J Autoimmun

Autoantibodies against the NineTeen complex and U5 RNP in systemic sclerosis

Authors - Jean-Baptiste Vulsteke
mouseWB

Nat Commun

Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance.

Authors - Yan-Ting Chen