• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66369-1-Ig (same clone as 66369-1-PBS)

    WB analysis using 66369-1-Ig (same clone as 66369-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66369-1-Ig (PGC1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66369-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66369-1-Ig (same clone as 66369-1-PBS)

    WB analysis of HeLa using 66369-1-Ig (same clone as 66369-1-PBS)

    WB result of PGC1a antibody (66369-1-Ig; 1:8000; room temperature for 1.5 hours) with wild-type and PGC1a knockout HeLa cells. This data was developed using the same antibody clone with 66369-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66369-1-Ig (same clone as 66369-1-PBS)

    IF Staining of HeLa using 66369-1-Ig (same clone as 66369-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PGC1a antibody (66369-1-Ig, Clone: 1C1B2 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66369-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PGC1a Monoclonal antibody, PBS Only

PGC1a Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66369-1-PBS

产品说明书

CloneNo. 1C1B2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IF/ICC, Indirect ELISA

别名

PPARGC1A, PGC 1v, PGC 1(alpha), PGC 1 alpha, Ligand effect modulator 6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

66369-1-PBS targets PGC1a in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
别名 PPARGC1A, PGC 1v, PGC 1(alpha), PGC 1 alpha, Ligand effect modulator 6
计算分子量 91 kDa
观测分子量 70 kDa, 100 kDa
GenBank蛋白编号NM_013261
基因名称 PGC1a
Gene ID (NCBI) 10891
RRIDAB_2828002
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UBK2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists an isoform in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.