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PGD Monoclonal antibody

PGD Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A10G4

Cat No : 67916-1-Ig

Print datasheet

Synonyms

6PGD, PGD, PGDH, phosphogluconate dehydrogenase

验证数据展示

  • WB analysis using 67916-1-Ig

    WB analysis using 67916-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67916-1-Ig (PGD antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HepG2 using 67916-1-Ig

    IF Staining of HepG2 using 67916-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PGD antibody (67916-1-Ig, Clone: 2A10G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inLNCaP cells, Jurkat cells, K-562 cells, Hela cells, HEK-293 cells
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67916-1-Ig targets PGD in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, WB, ELISA Application Description
Tested Reactivity Human
Immunogen PGD fusion protein Ag6871 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphogluconate dehydrogenase
Synonyms 6PGD, PGD, PGDH, phosphogluconate dehydrogenase
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 53 kDa, 45 kDa
GenBank Accession NumberBC000368
Gene Symbol PGD
Gene ID (NCBI) 5226
RRIDAB_2918670
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP52209
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PGD, also named as PGDH, belongs to the 6-phosphogluconate dehydrogenase family. PGD catalyses the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate in the context of the oxidative part of the pentose phosphate pathway. PGD is important for the production of NADPH, which is necessary for reductive biosynthesis, such as the formation of lipids and nucleotides, and the activity of enzymes involved in maintaining cell integrity, in combatting oxidative stress and in the first line of immunological defence. (PMID: 35234135)

实验方案

Product Specific Protocols
WB protocol for PGD antibody 67916-1-IgDownload protocol
IF protocol for PGD antibody 67916-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols