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验证数据展示

  • WB analysis using 67916-1-Ig

    WB analysis using 67916-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67916-1-Ig (PGD antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HepG2 using 67916-1-Ig

    IF Staining of HepG2 using 67916-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PGD antibody (67916-1-Ig, Clone: 2A10G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

PGD Monoclonal antibody

PGD Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67916-1-Ig

产品说明书

CloneNo. 2A10G4

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

6PGD, PGD, PGDH, phosphogluconate dehydrogenase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, Jurkat cells, K-562 cells, Hela cells, HEK-293 cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67916-1-Ig targets PGD in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 PGD fusion protein Ag6871 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphogluconate dehydrogenase
别名 6PGD, PGD, PGDH, phosphogluconate dehydrogenase
计算分子量 53 kDa
观测分子量 53 kDa, 45 kDa
GenBank蛋白编号BC000368
基因名称 PGD
Gene ID (NCBI) 5226
RRIDAB_2918670
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP52209
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PGD, also named as PGDH, belongs to the 6-phosphogluconate dehydrogenase family. PGD catalyses the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate in the context of the oxidative part of the pentose phosphate pathway. PGD is important for the production of NADPH, which is necessary for reductive biosynthesis, such as the formation of lipids and nucleotides, and the activity of enzymes involved in maintaining cell integrity, in combatting oxidative stress and in the first line of immunological defence. (PMID: 35234135)

实验方案

Product Specific Protocols
WB protocol for PGD antibody 67916-1-IgDownload protocol
IF protocol for PGD antibody 67916-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols