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验证数据展示

  • FC experiment of HepG2 using CL488-67916

    FC experiment of HepG2 using CL488-67916

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PGD (CL488-67916, Clone:2A10G4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL488-67916

    IF Staining of HepG2 using CL488-67916

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 PGD antibody (CL488-67916, Clone: 2A10G4 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated PGD Monoclonal antibody

PGD Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67916

产品说明书

CloneNo. 2A10G4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

PGDH, EC:1.1.1.44, 6-phosphogluconate dehydrogenase, decarboxylating, 6PGD, 2A10G4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67916-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67916 targets PGD in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 PGD fusion protein Ag6871 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphogluconate dehydrogenase
别名 PGDH, EC:1.1.1.44, 6-phosphogluconate dehydrogenase, decarboxylating, 6PGD, 2A10G4
计算分子量 53 kDa
观测分子量 53 kDa, 45 kDa
GenBank蛋白编号BC000368
基因名称 PGD
Gene ID (NCBI) 5226
RRIDAB_2923824
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP52209
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PGD, also named as PGDH, belongs to the 6-phosphogluconate dehydrogenase family. PGD catalyses the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate in the context of the oxidative part of the pentose phosphate pathway. PGD is important for the production of NADPH, which is necessary for reductive biosynthesis, such as the formation of lipids and nucleotides, and the activity of enzymes involved in maintaining cell integrity, in combatting oxidative stress and in the first line of immunological defence. (PMID: 35234135)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PGD antibody CL488-67916Download protocol
FC protocol for CL Plus 488 PGD antibody CL488-67916Download protocol
Standard Protocols
Click here to view our Standard Protocols