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PGM1 Monoclonal antibody, PBS Only

PGM1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, rabbit, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G5A8

Cat no : 66105-1-PBS

Print datasheet

Synonyms

Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1

验证数据展示

  • WB analysis using 66105-1-Ig (same clone as 66105-1-PBS)

    WB analysis using 66105-1-Ig (same clone as 66105-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66105-1-Ig (PGM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66105-1-Ig (same clone as 66105-1-PBS)

    IHC staining of human colon using 66105-1-Ig (same clone as 66105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PGM1 antibody included
  • IHC staining of human colon using 66105-1-Ig (same clone as 66105-1-PBS)

    IHC staining of human colon using 66105-1-Ig (same clone as 66105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PGM1 antibody included
  • IF Staining of HepG2 using 66105-1-Ig (same clone as 66105-1-PBS)

    IF Staining of HepG2 using 66105-1-Ig (same clone as 66105-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGM1 antibody (66105-1-Ig, Clone: 2G5A8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PGM1 Ready-To-Use IHC Kit. PGM1 primary antibody included.

产品信息

66105-1-PBS targets PGM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, rabbit, pig
Immunogen PGM1 fusion protein Ag7294 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name phosphoglucomutase 1
Synonyms Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC001756
Gene Symbol PGM1
Gene ID (NCBI) 5236
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36871
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.