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PGM1 Monoclonal antibody, PBS Only

PGM1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, rabbit, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G5A8

Cat No : 66105-1-PBS

Print datasheet

Synonyms

Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1

验证数据展示

  • WB analysis using 66105-1-Ig (same clone as 66105-1-PBS)

    WB analysis using 66105-1-Ig (same clone as 66105-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66105-1-Ig (PGM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66105-1-Ig (same clone as 66105-1-PBS)

    IHC staining of human colon using 66105-1-Ig (same clone as 66105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PGM1 antibody included
  • IHC staining of human colon using 66105-1-Ig (same clone as 66105-1-PBS)

    IHC staining of human colon using 66105-1-Ig (same clone as 66105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PGM1 antibody included
  • IF Staining of HepG2 using 66105-1-Ig (same clone as 66105-1-PBS)

    IF Staining of HepG2 using 66105-1-Ig (same clone as 66105-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGM1 antibody (66105-1-Ig, Clone: 2G5A8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PGM1 Ready-To-Use IHC Kit. PGM1 primary antibody included.

产品信息

66105-1-PBS targets PGM1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit, pig samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, rabbit, pig
Immunogen PGM1 fusion protein Ag7294 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name phosphoglucomutase 1
Synonyms Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC001756
Gene Symbol PGM1
Gene ID (NCBI) 5236
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36871
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.