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PGM1 Monoclonal antibody

PGM1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, rabbit, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G5A8

Cat no : 66105-1-Ig

Print datasheet

Synonyms

Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1

验证数据展示

  • WB analysis using 66105-1-Ig

    WB analysis using 66105-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66105-1-Ig (PGM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 66105-1-Ig

    IHC staining of human colon using 66105-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with PGM1 antibody included
  • IHC staining of human colon using 66105-1-Ig

    IHC staining of human colon using 66105-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with PGM1 antibody included
  • IF Staining of HepG2 using 66105-1-Ig

    IF Staining of HepG2 using 66105-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGM1 antibody (66105-1-Ig, Clone: 2G5A8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, MDA-MB-231 cells, HepG2 cells, pig liver tissue, rabbit liver tissue, rat liver tissue, mouse liver tissue, pig heart tissue, rabbit heart tissue
Positive IHC detected inhuman colon tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PGM1 Ready-To-Use IHC Kit. PGM1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66105-1-Ig targets PGM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, rabbit, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, rabbit, pig
Immunogen PGM1 fusion protein Ag7294 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name phosphoglucomutase 1
Synonyms Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC001756
Gene Symbol PGM1
Gene ID (NCBI) 5236
RRIDAB_2881504
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36871
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PGM1 antibody 66105-1-IgDownload protocol
IHC protocol for PGM1 antibody 66105-1-IgDownload protocol
IF protocol for PGM1 antibody 66105-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols