PGM1 Monoclonal antibody

PGM1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66105-1-Ig

产品说明书

CloneNo. 2G5A8

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, rabbit, pig

应用

WB, IHC, IF/ICC, ELISA

Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, MDA-MB-231 cells, HepG2 cells, pig liver tissue, rabbit liver tissue, rat liver tissue, mouse liver tissue, pig heart tissue, rabbit heart tissue
Positive IHC detected inhuman colon tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PGM1 Ready-To-Use IHC Kit. PGM1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66105-1-Ig targets PGM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, rabbit, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, rabbit, pig
免疫原 PGM1 fusion protein Ag7294 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoglucomutase 1
别名 Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1
计算分子量 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC001756
基因名称 PGM1
Gene ID (NCBI) 5236
RRIDAB_2881504
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP36871
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PGM1 antibody 66105-1-IgDownload protocol
IHC protocol for PGM1 antibody 66105-1-IgDownload protocol
IF protocol for PGM1 antibody 66105-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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