CoraLite® Plus 488-conjugated PGM1 Monoclonal antibody

PGM1 Monoclonal Antibody for IF/ICC
Cat No. CL488-66105

产品说明书

CloneNo. 2G5A8

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, rabbit, pig

应用

IF/ICC

Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PGM1 Ready-To-Use IHC Kit. PGM1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66105-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66105 targets PGM1 in IF/ICC applications and shows reactivity with human, mouse, rat, rabbit, pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat, rabbit, pig
免疫原 PGM1 fusion protein Ag7294 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoglucomutase 1
别名 Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1
计算分子量 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC001756
基因名称 PGM1
Gene ID (NCBI) 5236
RRIDAB_3084206
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP36871
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PGM1 antibody CL488-66105Download protocol
Standard Protocols
Click here to view our Standard Protocols
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