验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy PGM1 Ready-To-Use IHC Kit. PGM1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66105-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66105 targets PGM1 in IF/ICC applications and shows reactivity with human, mouse, rat, rabbit, pig samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat, rabbit, pig |
| Immunogen | PGM1 fusion protein Ag7294 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | phosphoglucomutase 1 |
| Synonyms | Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1 |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 61 kDa |
| GenBank Accession Number | BC001756 |
| Gene Symbol | PGM1 |
| Gene ID (NCBI) | 5236 |
| RRID | AB_3084206 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P36871 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PGM1 antibody CL488-66105 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |