CoraLite® Plus 488-conjugated PGM1 Monoclonal antibody
PGM1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, rabbit, pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2G5A8
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy PGM1 Ready-To-Use IHC Kit. PGM1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66105-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66105 targets PGM1 in IF/ICC applications and shows reactivity with human, mouse, rat, rabbit, pig samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, mouse, rat, rabbit, pig |
Immunogen | PGM1 fusion protein Ag7294 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | phosphoglucomutase 1 |
Synonyms | Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1 |
Calculated Molecular Weight | 61 kDa |
Observed Molecular Weight | 61 kDa |
GenBank Accession Number | BC001756 |
Gene Symbol | PGM1 |
Gene ID (NCBI) | 5236 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P36871 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 PGM1 antibody CL488-66105 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |