验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66105 targets PGM1 in IF/ICC applications and shows reactivity with human, mouse, rat, rabbit, pig samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human, mouse, rat, rabbit, pig |
免疫原 | PGM1 fusion protein Ag7294 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | phosphoglucomutase 1 |
别名 | Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1 |
计算分子量 | 61 kDa |
观测分子量 | 61 kDa |
GenBank蛋白编号 | BC001756 |
基因名称 | PGM1 |
Gene ID (NCBI) | 5236 |
RRID | AB_3084206 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P36871 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 PGM1 antibody CL488-66105 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |