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验证数据展示

  • WB analysis using 82965-1-RR

    WB analysis using 82965-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82965-1-RR (PGM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 82965-1-RR

    IHC staining of human stomach using 82965-1-RR

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82965-1-RR (PGM5 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of PC-3 using 82965-1-RR

    FC experiment of PC-3 using 82965-1-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug Anti-Human PGM5 (82965-1-RR, Clone:230206C7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82965-1-RR

    Affinity and Kinetic Characterization of 82965-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82965-1-RR against Human PGM5 were performed. The affinity constant is 0.719 nM.

PGM5 Recombinant antibody

PGM5 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 82965-1-RR

产品说明书

CloneNo. 230206C7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

PGMRP, Aciculin, 230206C7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, PC-3 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82965-1-RR targets PGM5 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 PGM5 fusion protein Ag34399 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphoglucomutase 5
别名 PGMRP, Aciculin, 230206C7
计算分子量 62 kDa
观测分子量62 kDa
GenBank蛋白编号NM_021965
基因名称 PGM5
Gene ID (NCBI) 5239
RRIDAB_3670711
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15124
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phosphoglucomutase-like protein 5 (also known as aciculin) is an enzyme encoded by the PGM5 gene. Gene functional studies show that PGM5 is similar to PGM1 but lacks enzymatic activity. PGM5 is tightly associated with the actin cytoskeleton and has primarily been investigated as an adhesion protein. It also functions as a cytoskeletal component of cell-matrix and cell-cell contacts in muscle and non-muscle cells. (PMID: 35819729)

实验方案

Product Specific Protocols
WB protocol for PGM5 antibody 82965-1-RRDownload protocol
IHC protocol for PGM5 antibody 82965-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols